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codon optimized version of the alk cds  (GenScript corporation)

 
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    GenScript corporation codon optimized version of the alk cds
    Codon Optimized Version Of The Alk Cds, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon-optimized+cds/codon+optimized+version+of+the+alk+cds/pmc12085853-365-6-8
    Average 90 stars, based on 1 article reviews
    codon optimized version of the alk cds - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: The Kernel Size-Related Quantitative Trait Locus qKW9 Encodes a Pentatricopeptide Repeat Protein That Aaffects Photosynthesis and Grain Filling
    Article Snippet: To verify this, a codon-optimized CDS (optimized by a web tool OptimumGene: https://www.genscript.com/codon_opt_pr.html ) was fused with GFP and driven by expression from the cauliflower mosaic virus 35S promoter.

    Article Title: qKW9 encodes a pentatricopeptide repeat protein affecting photosynthesis and grain filling in maize
    Article Snippet: To verify this, a codon-optimized CDS (optimized by a web tool - https://www.genscript.com/codon_opt_pr.html ) was fused with green fluorescent protein (GFP) and driven by expression from the cauliflowner mosaic virus 35S promoter.

    Virus:

    Article Title: The Kernel Size-Related Quantitative Trait Locus qKW9 Encodes a Pentatricopeptide Repeat Protein That Aaffects Photosynthesis and Grain Filling
    Article Snippet: To verify this, a codon-optimized CDS (optimized by a web tool OptimumGene: https://www.genscript.com/codon_opt_pr.html ) was fused with GFP and driven by expression from the cauliflower mosaic virus 35S promoter.

    Article Title: qKW9 encodes a pentatricopeptide repeat protein affecting photosynthesis and grain filling in maize
    Article Snippet: To verify this, a codon-optimized CDS (optimized by a web tool - https://www.genscript.com/codon_opt_pr.html ) was fused with green fluorescent protein (GFP) and driven by expression from the cauliflowner mosaic virus 35S promoter.



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    Image Search Results


    (a) De-MARylation activity of SARS-CoV-2 MD tested by immunoblotting (WB). SARS-CoV-2 MD was incubated with MARylated GST-hPARP10 CD at molar ratio 1:1 in absence and presence of each depicted compound and excess. Samples were obtained at the indicated times. Coomassie Blue (CB) stain was used for the total protein amount verification. These experiments were performed in triplicates. (b) Chemical structures of the used compounds (left to right) ADPr, remdesivir, GS-441524 and its chlorinated analogue. (c) Quantification results for the experiments from panel (a) in 100 (left) and 500 (right) times excess for each of the used compounds. For this procedure the bands from each experiment were quantified by Image Lab software. Plots and rate quantification were obtained by Origin2019b after summarizing the results from each independent experiment for the tested compounds.

    Journal: Journal of Molecular Biology

    Article Title: Binding adaptation of GS-441524 diversifies macro domains and downregulate SARS-CoV-2 de-MARylation capacity

    doi: 10.1016/j.jmb.2022.167720

    Figure Lengend Snippet: (a) De-MARylation activity of SARS-CoV-2 MD tested by immunoblotting (WB). SARS-CoV-2 MD was incubated with MARylated GST-hPARP10 CD at molar ratio 1:1 in absence and presence of each depicted compound and excess. Samples were obtained at the indicated times. Coomassie Blue (CB) stain was used for the total protein amount verification. These experiments were performed in triplicates. (b) Chemical structures of the used compounds (left to right) ADPr, remdesivir, GS-441524 and its chlorinated analogue. (c) Quantification results for the experiments from panel (a) in 100 (left) and 500 (right) times excess for each of the used compounds. For this procedure the bands from each experiment were quantified by Image Lab software. Plots and rate quantification were obtained by Origin2019b after summarizing the results from each independent experiment for the tested compounds.

    Article Snippet: The codon optimized cDNA sequence coding the catalytic domain hPARP10 (hPARP10 CD) residues 806-1025 (GenBank entry BC014229.2) was obtained from GenScript, (Piscataway, NJ) into a pGEX-4T-1 vector providing an N-terminal GST-fusion tag.

    Techniques: Activity Assay, Western Blot, Incubation, Staining, Software

    (a) De-MARylation activity of SARS-CoV-1, MERS-CoV and SARS-CoV-2 F360N MDs tested by immunoblotting (WB). MDs were incubated with MARylated GST-hPARP10 CD at molar ratio 1:1 in absence and presence of each depicted compound and excess. Samples were obtained at the indicated times. Coomassie Blue (CB) stain was used for the total protein amount verification. These experiments were performed in triplicates. (b) Quantification results for the experiments from panel (a) for SARS-CoV-1 (left), MERS-CoV (middle) and SARS-CoV-2 F360N mutant (right) MDs. For this procedure the bands from each experiment were quantified by Image Lab software. Plots and rate quantification were obtained by Origin2019b after summarizing the results from each independent experiment for the tested compounds.

    Journal: Journal of Molecular Biology

    Article Title: Binding adaptation of GS-441524 diversifies macro domains and downregulate SARS-CoV-2 de-MARylation capacity

    doi: 10.1016/j.jmb.2022.167720

    Figure Lengend Snippet: (a) De-MARylation activity of SARS-CoV-1, MERS-CoV and SARS-CoV-2 F360N MDs tested by immunoblotting (WB). MDs were incubated with MARylated GST-hPARP10 CD at molar ratio 1:1 in absence and presence of each depicted compound and excess. Samples were obtained at the indicated times. Coomassie Blue (CB) stain was used for the total protein amount verification. These experiments were performed in triplicates. (b) Quantification results for the experiments from panel (a) for SARS-CoV-1 (left), MERS-CoV (middle) and SARS-CoV-2 F360N mutant (right) MDs. For this procedure the bands from each experiment were quantified by Image Lab software. Plots and rate quantification were obtained by Origin2019b after summarizing the results from each independent experiment for the tested compounds.

    Article Snippet: The codon optimized cDNA sequence coding the catalytic domain hPARP10 (hPARP10 CD) residues 806-1025 (GenBank entry BC014229.2) was obtained from GenScript, (Piscataway, NJ) into a pGEX-4T-1 vector providing an N-terminal GST-fusion tag.

    Techniques: Activity Assay, Western Blot, Incubation, Staining, Mutagenesis, Software

    (a) De-MARylation activity of CHIKV, MAYV and VEEV MDs tested by immunoblotting (WB). MDs were incubated with MARylated GST-hPARP10 CD at molar ratio 1:1 in absence and presence of each depicted compound and excess. Samples were obtained at the indicated times. Coomassie Blue (CB) stain was used for the total protein amount verification. These experiments were performed in triplicates. (b) Quantification results for the experiments from panel (a) for CHIKV (left), MAYV (middle) and VEEV (right) MDs. For this procedure the bands from each experiment were quantified by Image Lab software. Plots and rate quantification were obtained by Origin2019b after summarizing the results from each independent experiment for the tested compounds.

    Journal: Journal of Molecular Biology

    Article Title: Binding adaptation of GS-441524 diversifies macro domains and downregulate SARS-CoV-2 de-MARylation capacity

    doi: 10.1016/j.jmb.2022.167720

    Figure Lengend Snippet: (a) De-MARylation activity of CHIKV, MAYV and VEEV MDs tested by immunoblotting (WB). MDs were incubated with MARylated GST-hPARP10 CD at molar ratio 1:1 in absence and presence of each depicted compound and excess. Samples were obtained at the indicated times. Coomassie Blue (CB) stain was used for the total protein amount verification. These experiments were performed in triplicates. (b) Quantification results for the experiments from panel (a) for CHIKV (left), MAYV (middle) and VEEV (right) MDs. For this procedure the bands from each experiment were quantified by Image Lab software. Plots and rate quantification were obtained by Origin2019b after summarizing the results from each independent experiment for the tested compounds.

    Article Snippet: The codon optimized cDNA sequence coding the catalytic domain hPARP10 (hPARP10 CD) residues 806-1025 (GenBank entry BC014229.2) was obtained from GenScript, (Piscataway, NJ) into a pGEX-4T-1 vector providing an N-terminal GST-fusion tag.

    Techniques: Activity Assay, Western Blot, Incubation, Staining, Software